FIG. 3.

iCre expression in kidneys of transgenic mice. (a) PCR analysis of genomic DNA isolated from the tail of 6-week-old podocin-iCreER^T2 transgenic mice crossed with R26R mice. Each lane represents DNA from a different mouse. The upper gel shows genotyping for podocin-iCre-ER^T2. The bottom gel exhibits genotyping for the presence of ROSA26 transgene. (b) RT-PCR for iCre in the kidneys treated with DNase I. PCR reactions were performed using templates from RT reactions with or without reverse transcriptase to control for DNA contamination (PCR control). Each lane represents DNA from a different mouse. Amplification of β-actin mRNA served as an additional control. (c) RT-PCR of iCre mRNA expression in extrarenal tissues from transgenic mice. Kidney tissue was used as control.