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. 2011 Jan 7;88(1):30–41. doi: 10.1016/j.ajhg.2010.11.012

Figure 5.

Figure 5

Reduced Apoptosis and Altered BMP Signaling in the Interdigital Mesenchyme of Smoc1 Null Mice

(A–D) NB staining of left hindlimbs at E13.5 (A and B) and E14.5 (C and D). In comparison to control embryos (WT and Smoc1Tp/+ littermates) (A and C), the number of NB-stained apoptotic cells in the interdigital mesenchyme of Smoc1Tp/Tp mice was dramatically reduced between digits 2 and 3 and digits 3 and 4 at both E13.5 and E14.5, and the webbing remained at a distal level (B and D, magenta asterisk).

(E–J) Whole-mount in situ hybridization of right hindlimbs at E12.5 (E, G, and I) and E13.5 (F, H, and J). At E12.5, interdigital expression of Bmp2, Bmp7, and Msx2 was profoundly delayed in the hindlimbs of Smoc1Tp/Tp mice, and their expression in the interdigital mesenchyme was apparently perturbed, even at E13.5 (magenta arrows). Scale bar represents 500 μm.