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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Acta Biomater. 2010 Sep 8;7(2):492–504. doi: 10.1016/j.actbio.2010.08.021

Figure 2.

Figure 2

Gross examination of proteoglycan matrix deposition by histological and immunohistochemical (IHC) evaluation for chondrocytes encapsulated in 10, 15, or 20% PEG gels and cultured for 4 weeks. Sections were stained for negatively charged glycosaminoglycans (S-GAGs) (red) using Safranin O/Fast green. Cell nuclei (dark purple) were counterstained using hematoxylin. For IHC, sections were using antibodies against chondroitin-6-sulfate (red), and aggrecan (red). Cell nuclei (blue) were counterstained using DAPI. Images were acquired by laser scanning confocal microscopy. Scale bars represent 50 μm.