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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Oct 12;1813(1):159–167. doi: 10.1016/j.bbamcr.2010.09.018

Figure 1. Hypoxia increases CAIX expression and activity in MDA-MB-231 cells.

Figure 1

Panel A: Total membranes were collected from control cells or cells exposed to DFO or hypoxia for 16 hours. Western blot analysis of CAIX expression was performed using the NB-100 antibody. This blot is typical of at least four independent experiments. C: Control; D: DFO (desferoxamine mesylate), H: Hypoxia. Panel B: Plasma membranes were isolated from control or hypoxic cells and CAIX activity was measured by 18O exchange. These data represent duplicate experiments.