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. Author manuscript; available in PMC: 2012 Jan 1.

Published in final edited form as: Biochim Biophys Acta. 2010 Oct 12;1813(1):159–167. doi: 10.1016/j.bbamcr.2010.09.018

MDA-MB-231 cells were exposed to normoxic (Panel A) or hypoxia (Panel B) conditions for 16 h. Total membranes were collected and extracted with 1% TX-100. Detergent-resistant membranes (lipid rafts) were separated from extracted protein by flotation on sucrose gradients (fractions 2 through 13 as numbered at the bottom of each panel). Expression of CAIX, GLUT1, caveolin and transferrin receptor is indicated. The values to the right of the figures represent the percent of the total pool that migrates in lipid rafts. TM = total membrane fraction; Rafts = lipid raft-containing membranes; TX-100 Soluble = membrane proteins extracted by TX-100. These data are representative of at least triplicate experiments.

MDA-MB-231 cells were exposed to normoxic (Panel A) or hypoxia (Panel B) conditions for 16 h. Total membranes were collected and extracted with 1% TX-100. Detergent-resistant membranes (lipid rafts) were separated from extracted protein by flotation on sucrose gradients (fractions 2 through 13 as numbered at the bottom of each panel). Expression of CAIX, GLUT1, caveolin and transferrin receptor is indicated. The values to the right of the figures represent the percent of the total pool that migrates in lipid rafts. TM = total membrane fraction; Rafts = lipid raft-containing membranes; TX-100 Soluble = membrane proteins extracted by TX-100. These data are representative of at least triplicate experiments.