Table 1.

Oligonucleotide sequences used to generate ISH RNA probes and in quantitative (qRT-PCR) and semi-quantitative RT-PCR (sqRT-PCR). For added specificity, at least one of the pck1 and pck2 oligonucleotides were anchored in either the 5′ or 3′ untranslated region (UTR). In pck1 Forward, 10 of 21 total nucleotides (underscored) are unique to pck1. In pck1 Reverse, 14 of 20 total (underscored) are unique to pck1. The pck1 primers were used in PCR and to generate gene-specific, digoxigenin-labeled RNA probes for in situ hybridization.

Gene	Forward	Reverse
pck 1	5′TCTCCATCCCTCCGCTCATCA3′ (5′URT)	5′ GGCCCAGCTGACTGCTCCT3′ (Exon 2)
pck2	5′ CAAACACTGGCATCTGCAT3′ (3′URT)	5′ TGGCCAGCTCAGGTA3′ (3′ UTR)
insa	5′GTTGGTCGTGTCCAGTGTAAGCACTAA3′	5′CCACCTCAGTTTCCTGGGCAGATTTA3′
insa	5′TCTCTGCCTGGATCGCAGTCTTCT3′	5′GGATCCGCATCTGCTGCCTCATAA3′
β actin	5′CCCCTCCATTGTTGGACGAC3′	5′TAGCCACGCTCGGTCAGGAT3′
insRa	5′CATAACCTGATGCAGATGTGCTGGCAT3′	5′TGCGTGTACGGCACATGCTCCTCAT3′
insRb	5′TTGCCGAGCCAGTGACTCACGAA3′	5′ACCAGTAAGAAAATGCAGATGGCTA3′