Table 4.

Transient kinetics analyses of Myo1c^1IQ-SAH and hearing mutants

Parameter	Units	Myo1c1IQ-WT		SAH-WT		SAH-R156W		SAH-V252A		SAH-T380M
		−Ca2+	+Ca2+	−Ca2+	+Ca2+	−Ca2+	+Ca2+	−Ca2+	+Ca2+	−Ca2+	+Ca2+
K′1k′+2 a	μM−1s−1	0.081	0.012	0.0195	0.0108	0.0555	0.026	0.023	0.015	0.123	0.0038
K′1	μM−1	1.97	0.602*	0.376	0.780	0.968	0.645	0.576	1.69*	1.30*	1.34*
k′+2	s−1	41.2	19.6	51.7	13.8	57.4	41.0	40.5	8.9	94.4	17.2
k′+α	s−1	2.0	2.7	2.59	3.99	2.95	7.7	1.55	3.03	0.67	4.41
K′α		0.11	0.115	0.11	0.238	0.2	0.22	0.09	0.15	0.1	0.13
K′aAD	μM	1.6	17.2	4.4	34.0	5.1	30.0	3.8	25.0	7.9	33.5
k′−AD	μM−1s−1	1.2	(1.2)	1.33	(1.33)	1.0	(1.0)	1.02	(1.0)	3.0	(3.0)
k′+AD a	s−1	1.9	20.6	5.8	45	4.4	30	3.8	25	30.0	85
K6K7	μM	3.3*	–	1.9	–	0.7	–	4.1	–	1.6*	–
k+6	s−1	6	5–20	~15	~7	6–7	~5	~8.5	~6.0	~5.8	~5.8
k+3+k−3	s−1	44.3	6.3	42.9	9.3	48.2	9.2	40.0	8.3 ± 0.4	44.4, fast 5.4, slow	9.09

Parentheses indicate values that are assumed to be independent of calcium. Asterisks indicate errors in the range 30–50%; all other errors are <20%

^aCalculated. K′_AD = K_app × (1 + 1/K′_α), k′_+AD = K′_AD × k′_−AD