Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2010 Nov 1;50(1):166–178. doi: 10.1016/j.freeradbiomed.2010.10.709

Fig. 9. Characterization of reduced cross-chain peroxide derived from oxidation of L4CL by LC-Iontrap MS.

Fig. 9

(A) MS2 of m/z 1514. The oxidized two side chains can be on the same glycerol backbone (9a) or different glycerol backbone (9b). Fragments m/z 761 and 695 are characteristic of 9a while m/z 711 and 745 are from 9b. (B) MS3 of m/z 1514 to 761, the fragments of m/z 465, 431, 295, and 329 indicate the presence of a hydroxyl group on one side chain and three hydroxyl groups on a neighboring side chain. (C) MS3 of m/z 1514 to 695, the fragments with m/z 279, 415, and 433 suggest two intact linoleoyl side chains. (D) MS4 of m/z 1514 to 761 to 329. (E) MS4 of m/z 1514 to 761 to 295, m/z 171 and 195 are characteristic of 9- and 13-HODE respectively. L4CL was oxidized using cytochrome c and H2O2 as described in details in the experimental section.