ELISA detection of B. neotomae LPS specific antibodies IgG, IgG1, and IgG2a antibodies in serum of mice vaccinated with gamma irradiated B. neotomae, B. neotomae/Bp26, and B. neotomae/SOD, or inoculated with saline. Serum samples were collected at 2 and 6 weeks after initial vaccination, and were diluted 1 in 200 and assayed for the presence of total antigen specific antibodies. Results are shown as mean ± standard deviation (n=4) of absorbance at 450 nm of the color developed.
* Significantly different from the corresponding saline group at week 2 (P<0.05).
** Significantly different from the corresponding saline group at week 6 (P<0.05).
ff Week 6 is significantly different from week 2 within each vaccine group (P<0.05).