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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2010 Oct 21;50(1):55–65. doi: 10.1016/j.freeradbiomed.2010.10.689

Fig. 5.

Fig. 5

Immunolocalization of the oxidative stress biomarker 4HNE in the small intestine of mice 8 h after acute whole-body irradiation with 8.5 Gy. (A) Intestinal section from unirradiated mice probed with a secondary (Alexa 488 goat antirabbit) antibody but no primary (rabbit anti-4HNE) antibody. (B) Section from unirradiated mice probed with both primary and secondary antibodies. (C) Section from irradiated mice probed with a secondary antibody only. (D) High levels of 4HNE were found in the intestine of irradiated mice probed with primary and secondary antibodies. Epithelial cells (e) lining the mucosa seemed to have greater amounts of 4HNE than the submucosal (s) or muscle (m) layers.