FIGURE 7.

ManLAM activation of MAPK-p38 regulates the PPARγ-mediated IL-8 response through activation of cPLA₂ in macrophages. MDMs were pretreated with the p38 inhibitor SB-203580 (5 μM) or DMSO for 30 min and subsequently stimulated with ManLAM (5 μg/ml) or left unstimulated for 3, 6, 9, and 24 h. Cell culture supernatants were analyzed for IL-8 production by ELISA (A); a representative experiment is shown. Mean ± SD of triplicate samples. n = 3. *p < 0.013; **p < 0.0003. Cell lysates were used to analyze the activation of p38 (B) and cPLA₂ (C) by Western blotting using phospho-specific Abs and reprobed for total p38 and cPLA₂ or actin. The Western blots shown are representative of three independent experiments. D, MDMs were pretreated with the cPLA₂ inhibitor MAFP (2.5 nM) for 30 min and subsequently stimulated with ManLAM (5 μg/ml) for the indicated times. Cell culture supernatants were analyzed for IL-8 production by ELISA; a representative experiment is shown. Mean ± SD of triplicate samples. n = 3. *p < 0.0024; **p < 0.0003.