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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: J Immunol. 2010 Jun 16;185(2):929–942. doi: 10.4049/jimmunol.1000866

FIGURE 9.

FIGURE 9

NF-κB mediates IL-8 production following BCG but not M. tuberculosis infection of human macrophages. MDMs were pretreated with the NF-κB inhibitor SN-50 (75 μg/ml) for 30 min and subsequently infected with M. tuberculosis H37Rv or BCG at an MOI of 5:1 or stimulated with LPS (100 ng/ml). The cell culture supernatants were analyzed for IL-8 (A) and TNF-α (B) production by ELISA. A and B are representative experiments (mean ± SD of triplicate samples; n = 3; *p < 0.0080; **p < 0.0006). C, In parallel, NF-κB inhibitor-treated cells were stimulated with ManLAM (5 μg/ml) for 6 and 24 h or with LPS (100 ng/ml) for 24 h. Cell culture supernatants were used to measure IL-8 production by ELISA. Shown is a representative experiment (mean ± SD of triplicate samples; n = 3; **p < 0.0006).