Fig. 2.

Barrier defect assays showing disturbance of outside-in barrier in Adam10-deficient mice. (A) Adam10 mutant mice (kept at 37°C) show pronounced water loss with subsequent death between 5 and 7 hours. Insets show the ventral side of control and mutant mice incubated at 37°C for 5 hours. (B) Transepidermal water loss (TEWL) measurements of control and Adam10-deficient mice; n=4 for each genotype. (C) Hematocrit analysis of blood from control (n=16) and Adam10-deficient mice (n=13). Results are mean±s.e.m. ^*P<0.05, ^***P<0.0005. (D) Epidermal loss of Adam10 does not affect the outside-in barrier: Toluidene Blue penetration assay of E14.5, E16.5 and E18.5 shows no time-course dependent difference of barrier formation between control and Adam10-deficient mice.