pTH-R identity is partially dependent on Nkx2.2. (A-H) The expression of Gad67 (A,B) and Ascl1 (E,F) are reduced (black arrowheads), whereas Vglut2 (C,D) and Ngn2 (G,H) are upregulated (white arrowheads) in the pTH-R (rostral thalamic progenitors) domain of Nkx2.2–/– embryos versus wild type (WT) at E12.5.