Fig. 1.

Repression of nanos1 translation in Xenopus oocytes is mediated by the ORF. (A) (Left) nanos1 transcripts were co-injected with [³⁵S]methionine into oocytes and incubated overnight at 18°C. β-globin (β-G) served as control. (Right) nanos1 and β-globin were translated in reticulocyte lysates. (B) Schematic of the transcripts tested and the experimental design. ORFs (uppercase) and UTRs (lowercase) were from nanos1 [X] or β-globin [B]. (C) RNAs with UTR substitutions (labeled in accordance with B) were assayed in oocyte extracts. Arrowheads mark the position of nanos1. See also Fig. S1 in the supplementary material.