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. Author manuscript; available in PMC: 2011 Jan 4.
Published in final edited form as: Eur J Immunol. 2009 Sep;39(9):2584–2595. doi: 10.1002/eji.200839031

Figure 6. Itk potentiates the TFII-I-driven transcriptional activity of the c-fos promoter under basal and growth factor-stimulating conditions.

Figure 6

(A) Wild type TFII-I-Δ-GST was expressed in the presence or absence of wild type ITK-Myc in COS-7 cells, along with a c-fos promoter luciferase construct and the levels of transcription measured by a luciferase reporter assay. The pEBG empty vector was used as a negative control in the assay. Transfected cells were either stimulated with EGF for 4 h (EGF columns) or remained untreated in serum-free medium (control columns). RLU: relative luciferase units. Transfections were performed in triplicate and the results presented as mean ± SD. (B) Western blot analysis of equivalent volumes (50µl) of the COS-7 whole cell lysates used in the luciferase reporter assay. TFII-I-Δ-GST protein levels were visualized using anti-GST Ab (top panel). The membrane was stripped and re-probed using anti-Itk Ab to monitor Itk-Myc protein levels (bottom panel). The results are representative of at least two experiments for (A) and (B).