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. 2001 Feb 27;98(5):2381–2386. doi: 10.1073/pnas.041603398

Figure 1.

Figure 1

(A) PS1 forms complexes with E-cadherin and γ-catenin. Extract of confluent MDCK cells in TNE buffer plus 1% digitonin (9) was treated with the antibodies indicated at the top of the figure, and the resulting IPs were probed on WBs with antibodies against the proteins indicated on the right. For reference, 20 μg of MDCK cell lysate was also probed. TR, transferrin receptor; PI-R222, rabbit 222 preimmune serum; I-R222, rabbit 222 antiserum; 33B10, anti-PS1/CTF monoclonal antibody. (B) Triton X-100 effects on PS1/β-catenin and PS1/E-cadherin complexes. Extracts of confluent MDCK cells in 1% digitonin were immunoprecipitated with antibodies against E-cadherin (E-cad) or β-catenin (β-cat), and the resulting IPs were washed in the presence (+) or absence (−) of 1% Triton X-100 (TX100). The remaining immunocomplexes were collected by centrifugation and probed on WBs with antibody R222 (PS1/NTF) or 33B10 (PS1/CTF).