Table 3.
Percentage of maturation and fertilization of swamp buffalo oocytes at 12, 18, 24, and 30 h after IVF.
| Time after IVF (h) | No. of oocytes (replicates) | No. of MII oocytes (%)* | No. of fertilization (%)** | Embryo development | ||
|---|---|---|---|---|---|---|
| (% of fertilized oocytes) | ||||||
| OA (%) | PF (%) | CC (%) | ||||
| 12 | 103 (9) | 63 (61.2) | 28.6a | 5 (27.8) | 13 (72.2) | 0 (0) |
| 18 | 97 (7) | 65 (67.0) | 50.8b | 3 (9.1) | 30 (90.9) | 0 (0) |
| 24 | 91 (7) | 59 (64.9) | 49.2b | 4 (13.8) | 25 (86.2) | 0 (0) |
| 30 | 87 (8) | 56 (64.4) | 48.2b | 4 (14.9) | 9 (33.3) | 14 (51.8) |
a,bWithin a column, differences were considered to be significant at P < .05. OA = oocyte activation; PF = pronuclear formation; CC = cell cleavage.
*Percentage is expressed in relation to the total number of oocytes used for in vitro maturation.
**Percentage is expressed in relation to the number of MII oocytes.