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. 2010 Dec 22;2011:386862. doi: 10.1155/2011/386862

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Copyright © 2011 Debbie M. Figueroa et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FISH mapping of maize CBM1.10 with sorghum BAC a0053G04. (a) A DAPI-stained image of spread pachytene chromosomes from OMAd1.36. (b) FITC image showing maize chromosome 1 direct-labeled with Alexa-488-dUTP-KWF total maize DNA. (c) Rhodamine image from direct-labeled sorghum BAC FISH signals (green arrows). (d) Cy-5 image of centromere FISH signal (blue arrowhead) with direct-labeled CentC. (e–g) Three-color overlay of the FITC (red), rhodamine (green), and Cy-5 (blue) images. (f) Straightened projection of the maize chromosome from panel (e). The locations of the centromere (blue arrowhead) and CBM1.10 BAC FISH signals (green bracket) are indicated along with the resulting cytogenetic locus name (boxed). (g) Straightened projections of six additional chromosomes aligned at their centromeres for comparison. All scale bars represent 5 μm.