Table 1.
CBM | Marker/locus | Probe | Accession number | Type | Enzyme(s) | MaizeGDB insert size (bp) |
Sequenced RFLP insert size |
---|---|---|---|---|---|---|---|
1.01 | tub1 | p-tub1 | AY987961 | Genomic | EcoRI/HindIII | 156 | 156 |
1.02 | umc157(chn) | p-umc157 | DQ123890 | Genomic | PstI | 1220 | 1250 |
1.03 | umc76(prob a) | p-umc76 | AY751079 | Genomic | PstI | 760 | 710 |
1.04 | asg45(ptk) | p-asg45 | AY771210 | Genomic | PstI | 350 | 332 |
1.05 | csu03 | p-csu03 | DQ123891 | cDNA | EcoRI/XhoI | 1200 | 1064 |
1.06 | umc67a | p-umc67 | AY771211 | Genomic | PstI | 650 | 644 |
1.07 | asg62 | p-asg62 | DQ001865 | Genomic | PstI | 500 | 465 |
1.08 | umc128 | p-umc128 | DQ123892 | Genomic | PstI | 740 | 755 |
1.09 | cdj2 | p-csu164 | DQ642431 | cDNA | EcoRI/XhoI | 700 | 466 |
1.10 | umc107a(croc) | p-umc107 | DQ642432 | Genomic | PstI | 1090 | 1105 |
1.11 | umc161a | p-umc161 | AY771212 | Genomic | PstI | 700 | 723 |
1.12 | bnl6.32a | p-bnl6.32 | Genomic | PstI | 2250 | ||
2.01 | bnl8.45ab | p-bnl8.45 | Genomic | PstI | 2100 | ||
2.02 | umc53a | p-umc53 | AY771214 | Genomic | PstI | 640 | 604 |
2.03 | umc06a | p-umc06 | AY771213 | Genomic | PstI | 590 | 608 |
2.04 | umc34 | p-umc34 | DQ001866 | Genomic | PstI | 970 | 934 |
2.05 | umc131 | p-umc131 | AY771215 | Genomic | PstI | 810 | 859 |
2.06 | umc255a | p-umc255 | DQ123893 | Genomic | PstI | 1050 | 1013 |
2.07 | umc005a | p-umc005 | AY771216 | Genomic | PstI | 850 | 830 |
2.08 | asg20 | p-asg20 | DQ123894 | Genomic | PstI | 550 | 337 |
2.09 | umc049a | p-umc049 | DQ123895 | Genomic | PstI | 630 | 627 |
2.10 | php20581b(tb) | p-php20581 | EU190456 | Genomic | PstI | 1400 | 1327 |
3.01 | umc032a | p-umc32 | DQ001867 | Genomic | PstI | 990 | 1019 |
3.02 | csu32 | p-csu32 | DQ123896 | Genomic | EcoRI/XhoI | 500 | 411 |
3.03 | asg24(gts) | p-asg24 | AY771217 | Genomic | PstI | 550 | 372 |
3.04 | asg48 | p-asg48 | DQ001868 | Genomic | PstI | 1600 | 1617 |
3.05 | umc102 | p-umc102 | DQ005498 | Genomic | PstI | 1010 | 1023 |
3.06 | bnl5.37ab | p-bnl5.37 | Genomic | PstI | 2300 | ||
3.07 | bnl6.16ab | p-bnl6.16 | Genomic | PstI | 2450 | ||
3.08 | umc17a | p-umc17 | AY771218 | Genomic | PstI | 850 | 840 |
3.09 | umc63a | p-umc63 | DQ123897 | Genomic | PstI | 620 | 881 |
3.10 | cyp1 | p-csu25 | DQ005499 | cDNA | EcoRI/XhoI | 1100 | 960 |
4.01 | agrr115c | p-agrr115 | Genomic | EcoRI/HindIII | 600 | ||
4.02 | php20725ab | p-php20725 | Genomic | PstI | 1650 | ||
4.03 | umc31a | p-umc31 | DQ123898 | Genomic | PstI | 550 | 582 |
4.04 | npi386(eks) | p-npi386 | DQ007988 | Genomic | PstI | 1200 | 1180 |
4.05 | agrr37bb | p-agrr37 | cDNA | EcoRI/HindIII | 949 | ||
4.06 | umc156a | p-umc156 | AY771219 | Genomic | PstI | 570 | 533 |
4.07 | umc66a(lcr) | p-umc66 | DQ007989 | Genomic | PstI | 1020 | 1036 |
4.08 | umc127c | p-umc127 | DQ642433 | Genomic | PstI | 1210 | 649 |
4.09 | umc52 | p-umc52 | DQ123899 | Genomic | PstI | 1500 | 824 |
4.10 | php20608a | p-php20608 | DQ007990 | Genomic | PstI | 780 | 1451 |
4.11 | umc169 | p-umc169 | DQ123900 | Genomic | PstI | 670 | 813 |
5.01 | npi409b | p-npi409 | Genomic | PstI | 710 | ||
5.02 | umc90 | p-umc90 | DQ642434 | Genomic | PstI | 1240 | 1226 |
5.03 | tub4 | p-tub4 | DQ007991 | cDNA | EcoRI/HindIII | 200 | 230 |
5.04 | bnl4.36 | p-bnl4.36 | DQ642435 | Genomic | PstI | 2300 | 2210 |
5.05 | csu93b | p-csu93 | DQ015673 | cDNA | EcoRI/XhoI | 800 | 677 |
5.06 | umc126a | p-umc126 | AY771220 | Genomic | PstI | 670 | 663 |
5.07 | umc108 | p-umc108 | DQ642436 | Genomic | PstI | 970 | 958 |
5.08 | bnl5.24aa | p-bnl5.24 | Genomic | PstI | 2500 | ||
5.09 | php10017 | p-php10017 | AY771221 | Genomic | PstI | 470 | 526 |
6.01 | umc85a | p-umc85 | AY772450 | Genomic | PstI | 600 | 561 |
6.02 | umc59aa | p-umc59 | Genomic | PstI | 930 | ||
6.03 | npi393 | p-G23A-06 | DQ015674 | Genomic | PstI | 1200 | 1249 |
6.04 | umc65a | p-umc65 | AY772451 | Genomic | PstI | 650 | 691 |
6.05 | umc21 | p-umc21 | DQ123901 | Genomic | PstI | 1050 | 1062 |
6.06 | umc38a | p-umc38 | DQ059316 | Genomic | PstI | 1010 | 1022 |
6.07 | umc132a(chk) | p-umc132 | DQ1238902 | Genomic | PstI | 500 | 472 |
6.08 | asg7a | p-asg7 | DQ642437 | Genomic | PstI | 550 | 353 |
7.01 | asg8(myb) | p-asg8 | DQ642438 | Genomic | PstI | 500 | 320 |
7.02 | asg34a(msd)a | p-asg34 | Genomic | PstI | 1350 | ||
7.03 | asg49 | p-asg49 | DQ059317 | Genomic | PstI | 400 | 400 |
7.04 | umc254 | p-umc254 | EU190457 | Genomic | PstI | 1050 | 1000 |
7.05 | umc245 | p-umc245 | AY772452 | Genomic | PstI | 700 | 665 |
7.06 | umc168 | p-umc168 | DQ059318 | Genomic | PstI | 1080 | 1072 |
8.01 | npi220a | p-G10F-01 | DQ059319 | Genomic | HindIII | 400 | 406 |
8.02 | bnl9.11a(lts)a | p-bnl9.11 | Genomic | PstI | 2400 | ||
8.03 | umc124a(chk) | p-umc124 | DQ059320 | Genomic | PstI | 1160 | 1162 |
8.04 | bnl7.08aa | p-bnl7.08 | Genomic | PstI | 2300 | ||
8.05 | bnl2.369a | p-bnl2.369 | cDNA | EcoRI | 700 | ||
8.06 | csu31aa | p-csu31 | cDNA | EcoRI/XhoI | 800 | ||
8.07 | npi268a | p-npi268 | DQ123903 | Genomic | PstI | 710 | 688 |
8.08 | npi414a | p-npi414 | AY772453 | Genomic | PstI | 870 | 893 |
8.09 | agrr21a | p-agrr21 | cDNA | EcoRI/HindIII | 899 | ||
9.01 | umc109 | p-umc109 | AY772454 | Genomic | PstI | 840 | 797 |
9.02 | bz1a | p-umc192 | cDNA | PstI | 1750 | ||
9.03 | wx1a | p-umc25 | cDNA | EcoRI | 2300 | ||
9.04 | csu147a | p-csu147 | cDNA | EcoRI/XhoI | 800 | ||
9.05 | umc95 | p-umc95 | AY772455 | Genomic | PstI | 680 | 660 |
9.06 | csu61a | p-csu61 | cDNA | EcoRI/XhoI | 500 | ||
9.07 | asg12 | p-asg12 | DQ123904 | Genomic | PstI | 700 | 647 |
9.08 | csu54ba | p-csu54 | cDNA | EcoRI/XhoI | 1400 | ||
10.01 | php20075a(gast) | p-php20075 | DQ059321 | Genomic | PstI | 1400 | 1311 |
10.02 | npi285a(cac)a | p-npi285 | Genomic | PstI | 1250 | ||
10.03 | umc130 | p-umc130 | DQ059322 | Genomic | PstI | 640 | 634 |
10.04 | umc64a | p-umc64 | Genomic | PstI | 710 | ||
10.05 | umc259a | p-umc259 | DQ123905 | Genomic | PstI | 550 | 579 |
10.06 | umc44a | p-umc44 | AY772456 | Genomic | PstI | 800 | 794 |
10.07 | bnl7.49a(hmd)a | p-bnl7.49 | Genomic | PstI | 2100 |
aFull length RFLP sequence not available.
bUnable to sequence with vector primers.
cPoly A tail at both ends of insert sequence.