Table 3.
Maize CBM-RFLP identified sorghum BACs.
| CBM | RFLP-probe | No. of BACS indentified | [contig] Member BACs scored | Southern blot comments |
|---|---|---|---|---|
| 1.01 | tub1 | 2 | [868] a0039M23 [154] a0053G19 |
Contiguous BACs not identified |
| 1.02 | umc157 | 3 | [154] a0045K08, a0064A06, a0055E08 | All BACs confirmed |
| 1.03 | umc076 | 4 | [193] a0046J07, a0086C09 [389] a0004B12 [?] a0071N02 |
BACs a0046J07, 86C09, and 71N02 confirmed |
| 1.04 | asg45 | 5 | [490] a0073P09 [536] a0080O07 [573] a0086J05, a0075I08 [967] a0036D16 |
Contiguous BACs not identified |
| 7 | [150] a0001K22 [187] a0061M20 [557] a0041B19 [?] a0029B11, a0036D16, a0043B12, a0052B23 |
|||
| 1.05 | csu003 | 4 | [179] a0001C04, a0026E17, a0052G17, a0094G22 | BACs a0001C04, 26E17, 94G22 confirmed |
| 1.06 | umc67a | 2 | [868] a0026H06, a0038A08 | |
| 1.07 | asg62 | 5 | [550] a0084J12 [537] a0084J14 [443] a0083B09 [542] a0095N15 [567] a0096N18 |
Contiguous BACs not identified |
| 1.08 | umc128 | 12 | [168] a0029P06, a0055L05, a0059H18, a0058G14, a0066G14, a0070C24, a0071B13, a0072H12, a0075H05, a0081P22, a0083A01, a0092C10 | Confirmation Hybe failed; only control seen |
| 1.09 | cjd2 | 8 | [159] a0011I10, a0023C11, a0039E21, a0056O22, a0081H24, a0091D22, a0093H06, a0093P21 | BACs 23C21-93P21 confirmed |
| 1.10 | umc107a | 8 | [154] a0006I08, a0016J24, a0041J08, a0041O17, a0043G04, a0046O22, a0053G19, a0061G12 | All BACs confirmed |
| 1.11 | umc161a | 5 | [147] a0014L19, a0035F09, a0058E04, a0074A24, a0092O22 | All BACs confirmed; the restriction fragments of BACs 14L19 & 35F09 were smaller than those of other BACs |
| 1.12 | bnl6.32 | 6 | [147] a0014L19, a0035F09, a0058E04 [?] a0014M19, a0067C08, a0006L16 |
|
| 3.01 | umc32a | 9 | [1] a0016H11, a0020E10, a0038G18, a0049H03, a0066I03, a0083B22, a0092J15, a0092J15, a0092K15 [293] a0048H03 [?] a0035E11, a0038G18, a0015L17, a0017E12 |
All BACs in contig 1 confirmed |
| 3.02 | csu32 | 6 | [1332] a0010D09, a0017F16, a0035I06, a0066B14, a0066B20 [?] a0015J10 |
|
| 3.03 | asg24a | Contiguous BACs not identified | ||
| 3.04 | asg48 | 0 | Contiguous BACs not identified | |
| 3.05 | umc102 | 9 | [898] a0016D10 [884] a0018B20 [394] a0055N13 [246] a0085B11 [894] a0083B12 [1257] a0090I03 [?] a007P18, a0012G07, a0027C12 |
Contiguous BACs not identified |
| 3.06 | bnl5.37a | 8 | [64] a0010K10, a0042I24, a0043H04, a0045D02, a0047F15, a0047J02, a0058O10, a0096N10 | |
| 3.07 | bnl6.16a | 7 (8) | [56] a0014M18, a0020L03, a0029M08 = a0029M07*, a0050D18, a0085M21 [143] a0051P09 [?] a0052D16 |
|
| 3.08 | umc17a | 6 | [49] a0002O18, a0041K14 [85] a0074H09 [?] a0074H10, a0096D08, a0035C01 |
|
| 3.09 | umc63a | 9 | [1292] a0021P11, a0025P06, a0046A02, a0043J05, a0044B15, a0055P13, a0029C04 [?] a0029C03 |
|
| 3.10 | cyp1 | 6 | [1118] a0029L19, a0067D01, a0078E10 [89] a0017J07, a0018N05 [?] a0096D16 |
All BACs in contig 1118 as well as BAC a0096D16 confirmed |
| 4.01 | agrr115a | PolyA at both ends | ||
| 4.02 | php20725a | 10 | [1] a0016H11, a0092J15, a0092K15, a0049H03 [1292] a0021P11, a0029C03, a0044B15, a0046A02, a0043J02, a0055P13 |
Confirmation Hybe failed |
| 4.03 | umc31a | 5 | [1320] a0001I16 [3] a0067O16 [?] a0057L09, a0084A23, a0007F05 |
Contiguous BACs not identified |
| 4.04 | npi386 | 7 | [232] a0002B15, a0019A12, a0035B24, a0058L10, a0071D21, a0078C04, a0082H03 | All BACs confirmed |
| 4.05 | agrr37b | 6 | [391] a0023C20, a0054H21, a0055K09, a0068N16, a0080D06, a0083G05 | All BACs except 55K09 confirmed |
| 4.06 | umc156a | 3 | [280] a0059B12, a0061G01, a0067K14, a0089D08 | |
| 4.07 | umc66a | Contiguous BACs not identified | ||
| 4.08 | umc127c | 3 | [290] a0082D10, a0059L11, a0033K19 | BACs 59L11 and 33K19 confirmed |
| 4.09 | umc32 | 6 | [352] a0030M10, a0045N24, a0050L19, a0056E02, a0069D03, a0072G04 | |
| 4.10 | php20608 | 6 | [313] a0008B07, a0015N19, a0019O20, a0026C03, a0063A16, a0064O16 | All BACs confirmed |
| 4.11 | umc169 | 5 | [316] a0012H04, a0017H01, a0038N14, a0040B11, a0094J01 | |
| 5.01 | npi409 | 2 | [483] a0006F01 [813] a0002O16 |
Contiguous BACs not identified |
| 5.02 | umc90 | 2 | [916] a0003P04 [1120] a0038C23 |
Contiguous BACs not identified |
| 4 | [154] a0045K08, a0055E08, a0064A06 [?] a0034N18 |
All BACs confirmed | ||
| 5.03 | tub4 | 0 | ||
| 5.04 | bnl4.36 | 8 | [118] a0025E21, a0024H23, a0046J09, a0045L08, a0041E24, a0069P23, a0058E11, a0051H03, a0052I15 [314] a0013B03, a0061I09, a0069K04 [385] a0045M19, a0078E12, a0078A08, a0066D12 |
All BACs confirmed except 13B03 |
| 5.05 | csu93b | 7 | [193] a0007P01, a0046J07, a0058O08, a0069L09, a0078B19, a0086C09 [382] a0009P04 |
|
| 5.06 | umc125a | 4 | [280] a0067K14, a0061G01, a0059B12, a0089D08 | All BACs confirmed |
| 5.07 | umc108 | 14 | [287] a0095P22, a0061G08, a0063D06, a0050A23, a0052I05, a0054L12, a0046N22 [1108] a0023P17, a0004F02, a006N04 [333] a0020D11, a0029N15 [1288] a0030E06, a0038E06 |
All BACs in contig 287 confirmed |
| 5.08 | bnl5.24a | 8 | [278] a0042O01, a0020K19, a0023D21, a0095O21, a0095O19, a0081C19, a0063M08, a0002O03 [1017] a0017F09, a0017E09, a0041G23, a0019G02, a0067B22 |
All BACs confirmed except 63M08 |
| 5.09 | php10017 | 6 | [275] a0028A23, a0033G16, a0043E09, a0057K02, a0060N20 [?] a0061I19 |
All BACs confirmed except 43E09 |
| 6.01 | umc085a | 10 | [426] a0006I03, a0009K09, a0072P24 [205] a0025E20, a0041O09, a0061M21, a0057H05, a0067N09, a0074E15, a0085D02 |
All BACs S. blot confirmed except 57H05 |
| 6.02 | umc059a | 9 | [366] a0004P17, a0006P16, a0019D19, a0054G04, a0063H16, a0069J20, a0080H11, a0082K02, a0089K05 | All BACs confirmed except 19D19 and 54G04 |
| 6.03 | npi393 | 6 | [759] a0004A06, a0017E10 [528] a0006D09, a0008G11, a0063A20 [?] a0039N21 |
All BACs confirmed |
| 6.04 | umc65a | Contiguous BACs not identified | ||
| 6.05 | umc21 | 5 | [382] a0006P21, a0030G09, a0036H03, a0039E04, a0061C05 | |
| 6.06 | umc38a | Contiguous BACs not identified | ||
| 6.07 | umc132a | 9 | [327] a0030L20, a0040J01, a0056F09, a0060H04, a0070I02, a0070K04, a0070K07, a0073H20 [?] a0020O17 |
All BACs except 20O17 confirmed with cross-Hybe to negative control |
| 6.08 | asg7a | 6 | [323] a0018I04, a0032J21, a0032K17, a0068H17, a0084B19 [486] a0015L19 |
Confirmation failed |
| 8.01 | npi220a | 6 | [22] a0027H19, a0063B11, a0080P02, a0084E01, a0096C21 [188] a0019O09 |
All BACs confirmed |
| 8.02 | bnl9.11 | 8 | [421] a0042P09, a0047M08, a0072L19, a0007N23, a0089I26 = 89I23* [908] a0074H18 [?] a0079F24, a0035C01 |
|
| 8.03 | umc124a | 7 | [6] a0014C07, a0017N03, a0040E17, a0078K17, a0002I13 = a0002I16(?), a0083P05 | |
| 8.04 | bnl7.08a | Contiguous BACs not identified | ||
| 8.05 | bnl2.369 | Contiguous BACs not identified | ||
| 8.06 | csu31a | 13 | [45] a0042J03, a0043C22, a0062K14, a0064A02 [233] a0089H10 = a0089G10* [359] a0078O19 = a0078N19* [915] a0085F10 = [866] 77F10* [?] a0035C01, a0044F14, a0053E10 = 51E10*, a0013N08, a0045C22, a0052D10, |
|
| 8.07 | npi268a | 10 | [61] a0033M15, a0073N21, a0039H13, a0045D11, a0059E23, a0065K05, a0068E24, a0072D01, a0078K07, a0089A17 | Confirmation failed; only positive control seen |
| 8.08 | npi414a | 9 | [58] a0007I19, a0007C07, a0033M05, a0073D02, [1019] a0002I19 = a0007I19* [1125] a0023G22 [223] a0046C21 [1278] a0090D06 [?] a0019G24 |
|
| 8.09 | agrr21 | Contiguous BACs not identified |
*Whenever BAC scoring was ambiguous, possible alternate identified BACs were also grown for S. blot verification.