Table 3.
Calorimetrically determined binding thermodynamics for A6 recognition of Tax-peptide variants.a
| Peptide | Δ H ° | Δ S ° | Δ G ° | KD | Δ H ° | Δ S ° | Δ G ° | KD |
|---|---|---|---|---|---|---|---|---|
| 4 °C | 37 °C | |||||||
| Native Tax b | 4.4 ± 0.2 | 43 ± 2 | −7.7 ± 0.2 | 0.88 ± 0.09 | −7.1 ± 0.1 | 4.7 ± 0.4 | −8.59 ± 0.04 | 0.9 ± 0.1 |
| Tax-Y5F4F | 6.0 ± 0.1 | 51 ± 1 | −8.2 ± 0.1 | 0.37 ± 0.05 | −6.4 ± 0.2 | 10 ± 1 | −9.5 ± 0.1 | 0.20 ± 0.03 |
| Tax-Y5F34FF | 7.5 ± 0.3 | 58 ± 1 | −8.7 ± 0.1 | 0.14 ± 0.03 | −5.4 ± 0.1 | 14 ± 1 | −9.7 ± 0.1 | 0.16 ± 0.02 |
a
Units of kcal/mol for ΔH° and ΔG° and cal/mol/K for ΔS°. KD values are in μM.
b
Data for native Tax from Armstrong et al. (2008); 4 °C data calculated from propagation of globally fitted parameters [13].