Table 1. Statistical comparison of the scotopic and photopic visual function of wild type, P14 vector treated, P35 vector treated, and untreated rd12 mouse eyes as measured by optomotor behavior.
Under scotopic conditions, P14 and P35 treatment produces acuity and contrast sensitivity similar to wild type animals and wild type, P14-treated and P35-treated eyes all have significantly better acuity and contrast sensitivity than untreated rd12 eyes. Under photopic conditions, only P14 treated eyes are not significantly different from wild type eyes; P35 treatment eyes did not differ significantly from rd12 untreated eyes for both photopic acuity and contrast sensitivity. Each mouse was tested for 4–6 trials per condition. Data from each animal (n=3–4) were then averaged to obtain the means for each condition. The standard deviation listed is the standard deviation of the individual mouse means.
| Scotopic Acuity | WT | p14 | p35 | Untreated | WT vs p14 | P = 0.5751 | Not significant |
| Number of mice | 4 | 4 | 3 | 3 | WT vs p35 | P = 0.4713 | Not significant |
| Mean | 0.355 | 0.3698 | 0.3899 | 0.07858 | WT vs Untreated | P< 0.0001 | *** |
| Standard Deviation | 0.03876 | 0.03125 | 0.07975 | 0.01313 | Untreated vs p14 | P< 0.0001 | *** |
| Untreated vs p35 | P = 0.0026 | ** | |||||
| Scotopic Contrast Sensitivity | WT | p14 | p35 | Untreated | WT vs p14 | P = 0.3924 | Not significant |
| Number of mice | 4 | 4 | 3 | 3 | WT vs p35 | P = 0.4486 | Not significant |
| Mean | 4.398 | 3.967 | 4.775 | 1.051 | WT vs Untreated | P< 0.0001 | *** |
| Standard Deviation | 0.739 | 0.3479 | 0.4946 | 0.03153 | Untreated vs p14 | P< 0.0001 | *** |
| Untreated vs p35 | P< 0.0001 | *** | |||||
| Photopic Acuity | WT | p14 | p35 | Untreated | WT vs p14 | P = 0.5467 | Not significant |
| Number of mice | 4 | 4 | 3 | 3 | WT vs p35 | P = 0.0016 | ** |
| Mean | 0.4993 | 0.4813 | 0.3346 | 0.3329 | WT vs Untreated | P = 0.0053 | ** |
| Standard Deviation | 0.03886 | 0.04046 | 0.02716 | 0.0555 | Untreated vs pi4 | P = 0.0091 | ** |
| Untreated vs p35 | P = 0.9644 | Not significant | |||||
| Photopic Contrast Sensitivity | WT | p14 | p35 | Untreated | WT vs p14 | P = 0.4718 | Not significant |
| Number of mice | 4 | 4 | 3 | 3 | WT vs p35 | P = 0.0094 | ** |
| Mean | 8.793 | 9.19 | 3.25 | 2.339 | WT vs Untreated | P = 0.0031 | ** |
| Standard Deviation | 2.79 | 6.925 | 0.9493 | 0.5343 | Untreated vs p14 | P = 0.022 | * |
| Untreated vs p35 | P = 0.2301 | Not significant |
P-values were calculated using an unpaired two-tailed t test.
p < 0.001,
p < 0.01,
p < 0.05.