Table 5.
Relative expression of folB and folP in L. plantarum harboring pNZ8148, pNZ7030, and pNZ7030 after 20 minutes and 4 hours following nisin induction and in the uninduced cultures
| 0 ng/ml nisin | 25 ng/ml nisin | ||
|---|---|---|---|
| time minutes | L. plantarum harboring | Average expression folB-folP | Average expression folB-folP |
| 20 | pNZ8148 sense | 1 | 1 |
| 20 | pNZ7030 sense | 64 | 584 |
| 20 | pNZ7031 antisense | 84 | 2864 |
| 240 | pNZ8148 sense | 1 | 1 |
| 240 | pNZ7030 sense | 1 | 38 |
| 240 | pNZ7031 antisense | 3 | 11 |
Expression values of the two folate genes, folB and folP, are normalized to groES, and are indicated as average expression folB-folP.