Table 7.
List of strains, constructed plasmids, and primers used in this study
| Material | Relevant features | Source of reference |
|---|---|---|
| Strains | ||
| L. lactis; NZ9000 | MG1363 pepN:nisRK, Cloning host | [44] |
| L. plantarum WCFS1 | Cloning host, genomic DNA isolation | [17] |
| L. plantarum NZ7100 | WCFS1 :nisRK, Cloning host | [46] |
| Plasmids | ||
| pNZ7021 | CmR, pNZ8148 derivative, nisin promoter replaced by pepN promoter | [1] |
| pNZ7026 | CmR, pNZ7021 derivative containing the folB, folP, folK, folE, xtp2 and folC2 gene cluster of L. plantarum WCFS1 | [18] |
| pNZ8148 | CmR, nisin regulated promotor | [44] |
| pNZ7030 | CmR, pNZ8148 derivative containing folB, folP, folK, folE, xtp2 and folC2 gene cluster of L. plantarum WCFS1 in the sense orientation | (this study) |
| pNZ7031 | CmR, pNZ8148 derivative containing folB, folP, folK, folE, xtp2 and folC2 gene cluster of L. plantarum WCFS1 in the antisense orientation | (this study) |
| Primers | ||
| LpfBnco-F | CTGGGATACCCATGGGCATGATTC | |
| LpfPkpn-R | CGTCAAAAGGTACCGGACTAATCATTATTCG | |
| pNis-F | TAGTCTTATAACTATACTGAC | |
| LpfB-R | CTTGCCATTCGGCGTCCCCTCCACCTCAATTTCC | |
| LpfBatg-F | ATGGGCATGATTCGAATTAATAATTTACG | |
| LpfP-xbatest | GAATTTAATTATTTGCGACGCCCAAT | |
| FQPCRfolBS | CCTATCGAAACCAAGGTTCAACA | |
| RQPCRfolBS | ACAAATTCATCGACCACGTTACG | |
| FQPCRfolBAS | TCAACTTGTATGAATGGGTCGTTACA | |
| RQPCRfolBAS | CGTTCACGAGACCATCAATTACG | |
| FQPCRFPS | CATTATTAACGATGTGAACGCCTTT | |
| RQPCRFPS | CGCGACTGTCAGCCATCAAT | |
| FQPCRfPAS | CTAACAGCGTAATCAATTGCTTGGT | |
| RQPCRfPAS | CTTAAGGGTGGCCGGATCA | |
| groES-fo(2) | CCCAAAGCGGTAAGGTTGTT | |
| groES-re(2) | CTTCACGCTGGGGTCAACTT | |
| pfk-fo1 | TCCAGGGACGATCGATAATGA | |
| pfk-re1 | GCTTGCACGTTGGTGTTGAAC |