Figure 3.

Mapping of m24 mAb to residues 1–254 of the β2 subunit. The indicated human/mouse β2 chimeras and mutants were cotransfected with human αL into 293T cells (11). The transfectants were stained with m24 mAb or TS1/22 mAb to αL followed by immunofluorescence flow cytometry. m24 recognition was measured as specific mean fluorescence intensity and quantitated as a percentage of total αLβ2 expression defined by staining with TS1/22 mAb. Results are the mean and SD of three independent experiments.