Table 1.
Reactivity of antibodies with LFA-1 cysteine substitution mutants
| mAb | % Wild-type binding
|
||||||
|---|---|---|---|---|---|---|---|
| K287C/K294C
|
L289C/K294C
|
K287C 293T | L289C 293T | K294C 293T | |||
| 293T | K562 | 293T | K562 | ||||
| BL5 | 92 ± 11 | 92 | 86 ± 16 | 98 | 93 | 92 | 88 |
| F8.8 | 94 | 102 | 84 | 94 | 96 | 100 | 95 |
| CBR LFA-1/9 | ND | 85 | ND | ND | ND | ND | ND |
| TS2/6 | 85 ± 6 | 89 | 79 ± 3 | 96 | 91 | 88 | 92 |
| May.035 | 93 ± 8 | 93 | 82 ± 14 | 101 | 96 | 95 | 106 |
| TS1/11 | 94 | 96 | 94 | 105 | ND | ND | ND |
| TS1/12 | 89 | 87 | 102 | 106 | 99 | 104 | 94 |
| TS1/22 | 96 ± 12 | 93 | 91 ± 8 | 110 | 103 | 96 | 92 |
| TS2/14 | 86 ± 9 | 95 | 83 ± 11 | 103 | 103 | 100 | 103 |
| 25.3.1 | 93 | 88 | 91 | 86 | ND | ND | ND |
| CBR LFA-1/1 | 44 ± 1 | 56 | 96 ± 8 | 118 | 86 | 93 | 89 |
| S6F1 | 89 | 97 | 95 | 86 | 98 | 86 | 92 |
| TS1/18 | 100 ± 10 | 97 | 96 ± 5 | 106 | 88 | 87 | 108 |
| YFC51 | 103 | 101 | 95 | 111 | ND | ND | ND |
| CLBLFA-1/1 | ND | 96 | ND | 101 | ND | ND | ND |
| May.017 | ND | 109 | ND | 111 | ND | ND | ND |
| 6.5e | ND | 84 | ND | 96 | ND | ND | ND |
| CBR LFA-1/7 | 95 | 95 | 92 | 97 | ND | ND | ND |
| CBR LFA-1/2 | ND | 86 | ND | 86 | ND | ND | ND |
| YTA-1 | ND | 111 | ND | 108 | ND | ND | ND |
Wild-type or mutant αL and wild-type β2 were coexpressed transiently on the surface of 293T cells or stably on K562 cells. Reactivity with antibodies was determined by flow cytometry. Mean fluorescence of each antibody binding was normalized to the mean fluorescence of mAb TS2/4 binding. The results are expressed as percent of wild-type binding ± difference from the mean. For some antibodies, only one experiment was done. ND, not determined.