Table 4.

m24 mAb maps to residues R122 and E175 of the β2 subunit

β2 subunit mutation	m24 binding, % of TS1/22
Human wild type	60.0  ± 1.7
Mouse wild type	1.7  ± 1.9
R122N	1.8  ± 1.5
R133Q	54.1  ± 3.0
R133Q/H332Q	54.6  ± 2.9
R133Q/N339Y	59.8  ± 1.7
R133Q/H332Q/N339Y	53.6  ± 2.0
D163E	54.4  ± 2.2
D163E/E175A	0.3  ± 0.3
E175A	1.7  ± 0.8
N190D	54.1  ± 2.6
L270M	73.3  ± 3.3
A290S	67.8  ± 2.6
S302K/R303K	66.6  ± 1.9
E325D	59.7  ± 1.4
H332Q	66.5  ± 1.2
N339Y	70.9  ± 2.0

m24 binding to αLβ2 transfectants was determined as described in Fig. 3.