Table 1.

Coefficients of variation for the real-time PCR for PPV-NS1 performed for four concentrations of the standard plasmid at one time (one block with four replications per concentration) or at four different times (four blocks).

Within one block or among four blocks	Concentration of standard plasmid (copies/μL)	n	Ct (mean)	S.D.	CV (%)
Within	6.0 × 108	4	17.67	0.38	2.15
Within	6.0 × 106	4	24.38	0.53	2.17
Within	6.0 × 104	4	30.23	0.44	1.46
Within	6.0 × 102	4	37.18	0.36	0.97
Among	6.0 × 108	4	17.35	0.04	0. 21
Among	6.0 × 106	4	24.30	0.14	0. 57
Among	6.0 × 104	4	30.83	0.14	0. 46
Among	6.0 × 102	4	37.80	0.16	0. 42