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. 2010 Nov 30;10:306. doi: 10.1186/1471-2180-10-306

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Copyright ©2010 Almagro-Moreno et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Vibrio Pathogenicity Island-2 (VPI-2) ORFs and primers used in this study. A. Schematic representation of VPI-2. Small black vertical arrows mark ORFs VC1758 (IntV2), VC1785 (VefA), or VC1809 (VefB). Block arrows represent ORFs and direction of transcription. Black arrows represent core genome ORFs (VC1757 and VC1810) present in all V. cholerae isolates; red arrow indicates an integrase, purple arrows indicate type 1 restriction modification ORFs, green arrows represent sialic acid catabolism, transport and regulator ORFs, blue arrow indicates sialidase/neuraminidase and yellow arrows represent transposase and Mu-phage like proteins. B. Schematic of VPI-2 excision mechanism and primer pair VPI2attF and VPI2attR used to detect the VPI-2 attB locus after excision of the entire region.