Table 2.
Oligonucleotide primers used in this study.
| Oligo name | Sequence (5'-3'). Restriction site underscored | Target gene |
|---|---|---|
| SOE PCR | ||
| VC1785A | GAGCTCAATGGTGCATCGGCATATTCT | vefA (A-D) |
| VC1785B | CAGCGATGATGGCGTGATTA | |
| VC1785C | TAATCACGCCATCATCGCTGGGATGT TCTCCTATGTCTTGT | |
| VC1785D | TCTAGACGCGCACATAACGCTGTTC | |
| VC1809A | CTGCAGTGAGAGCAAGGGAAGTGATCGT | vefB(A-D) |
| VC1809B | CGAGAGCTGGTTTACTTGTGTG | |
| VC1809C | CACACAAGTAAACCAGCTCTCGGTGCATGATTGTCAAGTCATGCA | |
| VC1809D | GAGCTCGATGGCTATGAATTAGCTCAGGA | |
| Flanking | ||
| VC1785FF | GATGCTTCTATTACTCGGTT | vefA (F-R) |
| VC1785FR | TCACCGCTGCTGCGTTAA | |
| VC1809FF | GATTGATAGTAACAACACGCG | vefB (F-R) |
| VC1809FR | GTAATGCGCTATTGCTAAGTG | |
| VPI2attF | AGAGTGAAAGTCGCCAAAGC | attB (F-R) |
| VPI2attR | GGGTGCAATTTCGCATGTTGC | |
| Complements | ||
| VC1758CF | GAGCTCGAGTCCTCATGCTCTAGCCAG | intV2 (F-R) |
| VC1758CR | TCTAGAGGCATGCTGGTGTGTTACTAC | |
| VC1785CF | GAGCTCGCTTTGAATATAGGTAAGGGACTG | vefA (F-R) |
| VC1785CR | TCTAGACTATAGTACATGACGCATGTATAATC | |
| Real-Time PCR | ||
| VPI2attBQF | GATTCGGTGAGTTGTCCGAGT | attB (VPI-2) (F-R) |
| VPI2attBQR | GTGTTGGTGCAATGCTCAGTC | |
| VC1758QF | TGCATGATCTTATACTCACCG | intV2 (F-R) |
| VC1758QR | ACGTGCTCGCGGTTCATCTTC | |
| VC1785QF | CCGCACTAAGCCGTTCAGCAA | vefA (F-R) |
| VC1785QR | CCATCCACTCATCCACTTCGC | |
| VC1809QF | CTGAGAGGTGTGAATATGCCAG | vefB (F-R) |
| VC1809QR | GTGATCCGTTGAGCAATCCAC | |
| VC0432QF | TGTATGATATTGCGCCTGTCACAC | mdh(F-R) |
| VC0432QR | CCAGAACCACATCCGCACCTTC |
Underlined sequence represents restriction enzyme sites SacI, XbaI and Pst1.