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Methylation specific primer (MSP) pairs.
| Primer pairs | Forward primer | Reverse primer | Fragment size (bp) |
|---|---|---|---|
| Methylated | GTTGTTATTGGTTAGGAAGTTTTCG | GCGTCTTAAAATAACCACCGTC | 114 |
| Unmethylated | TTGTTATTGGTTAGGAAGTTTTTGG | AACACATCTTAAAATAACCACCATC | 116 |
| Actb | GGAAATCGTGCGTGACATTA | AGGAAGGAAGGCTGGAAGAG | 183 |
Methylation specific primer (MSP) pairs for Gpx3 used in the methylation specific PCR. The gene Actb was used as control.
