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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arthritis Rheum. 2011 Jan;63(1):212–218. doi: 10.1002/art.30083

Figure 1. HIg-induced cytokine production and effect of IL-10.

Figure 1

CD14+ monocytes were purified from healthy donor and SLE patient blood. All experiments were done in pairs. Control and SLE monocytes were treated with HIg (325 μg/ml), in the presence or absence of interleukin-10 (IL-10) (20 ng/ml). All 17 experiments were done in pairs. Culture supernatants were harvested after 8 hours of incubation. Suppression rate of cytokine production by adding exogenous IL-10 was compared between control and SLE. A, TNFα production was measured by ELISA. B, IL-6 production was measured by ELISA. C, IL-10 production was measured by ELISA. D, Control and SLE monocytes were treated with HIg (325 μg/ml) in the presence or absence of IL-10 (20 ng/ml). RNA lysates were extracted after 3 hours of incubation. IL-6 mRNA was measured using RT-PCR, with results normalized to the values for GAPDH. Suppression of IL-6 gene expression by IL-10 was compared between control and SLE. * p=0.01 and ** p=0.002