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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arthritis Rheum. 2011 Jan;63(1):53–62. doi: 10.1002/art.30081

Figure 1.

Figure 1

Citrullination renders fibrinogen a more potent inducer of macrophage TNF production. RAW 267.4 macrophages A, and murine peritoneal macrophages B, were stimulated with native fibrinogen (nFb) or citrullinated fibrinogen (cFb) at the indicated concentrations (μg/ml). C, RAW 267.4 macrophages were pretreated with the endotoxin inhibitor Polymyxin B (PMB; 10 μg/ml) for 30 minutes and then stimulated with cFb, CpG oligodeoxynucleotides (CPG ODN; 1 μg/ml), or lipopolysaccharide (LPS; 100 ng/ml). TNF levels in culture supernatants following 16–18 hours of stimulation were measured by ELISA. Results are representative of experiments performed at least twice. Values are the mean and SEM of triplicate cultures.