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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arthritis Rheum. 2011 Jan;63(1):53–62. doi: 10.1002/art.30081

Figure 2.

Figure 2

Citrullinated fibrinogen induces macrophage TNF production via TLR4. A, Peritoneal macrophages from TLR4-deficient or wild-type (WT) B6 mice were stimulated with citrullinated fibrinogen (cFb; 50 μg/ml) or lipopolysaccharide (LPS; 100 ng/ml). B, RAW 267.4 macrophages were pretreated with the TLR4-inhibitor CLI-095 (0.5 μg/ml) or vehicle and then stimulated with increasing concentrations of cFb, with the TLR4 agonist LPS (100 ng/ml), or with the TLR9 agonist CpG oligodeoxynucleotides (CPG ODN; 1 μg/ml). TNF levels in culture supernatants following 16–18 hours of stimulation were measured by ELISA. Results are representative of experiments performed at least twice. Values are the mean and SEM of triplicate cultures.