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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arthritis Rheum. 2011 Jan;63(1):53–62. doi: 10.1002/art.30081

Figure 3.

Figure 3

Citrullinated-fibrinogen-containing immune complexes co-stimulate macrophage TNF production. A, RAW 267.4 macrophages were stimulated with citrullinated fibrinogen (cFb; 10μg/ml) alone or with cFb-containing immune complexes (cFb-IC) generated by incubating cFb (10μg/ml) with polyclonal anti-Fb antibody (50μg/ml). Citrullination buffer alone and lipopolysaccharide (LPS, 100 ng/ml) were used as negative and positive controls, respectively. B, RAW 267.4 macrophages were stimulated with Fb-IC generated using a concentration (3.12μg/ml) of native Fb (nFb) or cFb that alone elicits negligible macrophage TNF production, with nFb or cFb alone (3.12 μg/ml), or with anti-Fb antibody alone. C, RAW 267.4 cells were stimulated with cFb alone (10μg/ml) or cFb (10 μg/ml) incubated with normal polyclonal IgG (50μg/ml). TNF levels in culture supernatants following 16–18 hours of stimulation were measured by ELISA. Results are representative of experiments performed at least twice. Values are the mean and SEM of triplicate cultures.