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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arthritis Rheum. 2011 Jan;63(1):53–62. doi: 10.1002/art.30081

Figure 5.

Figure 5

cFb stimulate human macrophages to produce TNF production. A, Plasma samples from patients with ACPA-positive RA or from patients with osteoarthritis (OA) were assayed by ELISA for reactivity against native fibrinogen (nFb) and citrullinated fibrinogen (cFb). Values are O.D. for singlicate measures. B, Human monocyte-derived macrophages were stimulated with increasing concentrations of cFb in solution for 18 hours. For assessment of TLR4 dependence, cells were pretreated with TLR4-inhibitor CLI-095 (1.0 ug/ml) before adding cFb. Results are representative of experiments performed at least twice. Values are the mean and SEM of duplicate cultures.