Fig. 5.

Expression of neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP), and 2',3'-cyclic nucleotide 3'-phosphodiesterase monoclonal antibody (CNPase) in rats with pMCAO and in sham operated control rats. (A) Immunoblot was reacted with affinity purified anti-NeuN, anti-GFAP, and anti-CNPase antibodies, revealing 46-48, 50, and 46 KDa products. (B) Densitometric analysis revealed that focal cerebral ischemia produced a time-dependent decrease of NeuN (3 h: 52±6%; 6 h: 38±12%; 24 h: 29±8%, n=5,^*P<0.05) and GFAP (3 h: 69±5%; 6 h: 56±12%; 24 h: 53±14% n=5 ^*P<0.05) in the penumbra as compared with sham-operated controls. However, the expression of CNPase in the penumbra was not changed after pMCAO.