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Analysis of parasite loads in sand flies infected with the lines described in Fig. 3. qPCR was used to determine parasite DNA content in midguts from females with late-stage infections (10–12 days PBM). Thirty to 40 midguts were analysed for each line.A. Non-significant differences among FVI, KO and Kin parasites in P. papatasi, P = 0.8796, anova, Kruskal–Wallis test.B. Significant differences among FVI, +SHERP and +HASPB parasites in P. papatasi, P < 0.0001, anova, Kruskal–Wallis test.
