Fig. 6.

Expression of HASPB, SHERP and metacyclic LPG in promastigote stages of L. major FVI dissected from P. papatasi at 12 days PBM. Indirect immunofluorescence analysis was carried out with antibodies specific for each marker: a–d, 3F12 antibody for metacyclic LPG; e–h, anti-HASPB; i–l, anti-SHERP. Images a, b: 3F12-positive metacyclic promastigotes (MP); image c: 3F12-negative short promastigote (SP); image d: 3F12-negative SP and elongated nectomonad (EN); images e, f: HASPB-positive MPs; image g: HASPB-negative SP; image h: HASPB-negative EN; image i: SHERP-positive MP; image j, SHERP-positive SP; image k, SHERP-negative SP; image l, SHERP-negative EN. Size bars = 10 µm.