Fig. 7.

Expression of HASPB, SHERP and metacyclic LPG in promastigote stages of the parasite lines described in Fig. 3. Gut smears obtained by dissection of infected sandflies at day 9 and 12 PBM were analysed by indirect immunofluorescence as described in Experimental procedures. Promastigotes were measured and their reaction with antibodies was evaluated using Image J software. The % of metacyclic promastigotes of each line is shown, staining either positive (open box) or negative (closed box) with (A) 3F12 antibody for LPG; (B) anti-HASPB; (C) anti-SHERP. Recognition of short promastigotes with anti-SHERP is shown in (D). In all cases (A–D), the differences between strains were significant, as measured by chi-square test. [(A) χ² = 10.339, d.f. = 3, P = 0.016, (B) χ² = 29.160, d.f. = 4, P < 0.0001, (C) χ² = 27.914, d.f. = 4, P < 0.0001, (D) χ² = 23.840, d.f. = 4, P < 0.0001.]