Table 2.
Dimensions of the morphological forms of the lines described in Fig. 3 during development in P. papatasi at 2, 5, 9 and 12 days PBM.
| Body length |
Body width |
Flagellar length |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Days PBM | Leishmania strain | Morphological form | n | Mean (SD) (µm) | Range (µm) | Mean (SD) (µm) | Range (µm) | Mean (SD) (µm) | Range (µm) |
| 5 | FVI | EN | 230 | 19.0(3.3) | 14.9–34.5 | 1.5(0.5) | 0.7–3.3 | 18.3(2.4) | 13.3–25.5 |
| SP | 74 | 11.5(1.9) | 5.0–13.5 | 1.9(0.6) | 0.7–4.5 | 14.4(2.9) | 5.0–19.9 | ||
| MP | 13 | 8.1(1.6) | 5.0–9.9 | 1.6(0.1) | 1.5–1.7 | 17.8(2.4) | 14.9–21.6 | ||
| RF | 3 | 4.4(0.9) | 3.3–5.0 | 2.2(0.9) | 1.6–3.3 | 13.8(3.5) | 10.0–16.6 | ||
| Total | 320 | 16.7(4.8) | 3.3–34.5 | 1.6(0.6) | 0.7–4.5 | 17.3(3.0) | 5.0–25.5 | ||
| KO | EN | 149 | 19.7(3.4) | 15.0–30.0 | 1.4(0.3) | 0.7–1.5 | 17.2(2.4) | 12.0–25.5 | |
| SP | 10 | 12.1(1.1) | 10.5–13.5 | 1.5(0.0) | 1.5–1.5 | 13.5(2.5) | 9.0–18.0 | ||
| MP | 1 | 4.5 | 1.5 | 15.0 | |||||
| Total | 160 | 19.1(3.9) | 4.5–30.0 | 1.4(0.3) | 0.7–1.5 | 17.0(2.6) | 9.0–25.5 | ||
| Kin | EN | 122 | 18.3(2.4) | 15.0–25.5 | 1.4(0.5) | 0.7–3.0 | 17.1(2.2) | 9.0–24.0 | |
| SP | 35 | 12.3(1.3) | 9.0–13.5 | 1.7(0.6) | 0.7–3.0 | 14.1(2.6) | 9.0–21.0 | ||
| MP | 2 | 8.25(1.0) | 7.5–9.0 | 1.1(0.5) | 0.7–1.5 | 18.7(1.1) | 18.0–19.5 | ||
| Total | 160 | 16.8(3.6) | 7.5–25.5 | 1.4(0.5) | 0.7–3.0 | 16.4(2.6) | 9.0–24.0 | ||
| +HASPB | EN | 156 | 20.6(2.9) | 14.9–28.2 | 1.7(0.3) | 0.8–3.3 | 20.2(2.3) | 14.9–26.6 | |
| SP | 4 | 11.2(1.6) | 9.9–13.3 | 1.7(0.0) | 1.7–1.7 | 16.2(5.5) | 10.0–21.6 | ||
| Total | 160 | 20.4(3.2) | 9.9–28.2 | 1.7(0.3) | 0.8–3.3 | 20.1(2.5) | 10.0–26.6 | ||
| +SHERP | EN | 151 | 19.0(2.4) | 14.9–24.9 | 1.7(0.4) | 0.8–3.3 | 18.3(2.0) | 13.3–24.9 | |
| SP | 9 | 11.8(2.2) | 8.3–13.3 | 1.7(0.0) | 1.7–1.7 | 15.5(2.9) | 13.3–19.9 | ||
| Total | 160 | 18.6(2.9) | 8.3–24.9 | 1.7(0.4) | 0.8–3.3 | 18.1(2.2) | 13.3–24.9 | ||
| 9 | FVI | EN | 64 | 16.7(2.6) | 15.0–25.5 | 1.6(0.4) | 0.7–3.0 | 17.0(2.5) | 13.5–24.0 |
| SP | 183 | 10.6(2.2) | 4.5–13.5 | 1.6(0.5) | 0.7–3.3 | 13.3(2.9) | 4.5–21.6 | ||
| MP | 66 | 7.3(1.7) | 4.5–12.0 | 1.5(0.4) | 0.7–3.0 | 16.3(3.3) | 10.0–24.0 | ||
| RF | 7 | 4.0(0.7) | 3.3–5.0 | 4.1(0.7) | 3.3–5.0 | 14.1(5.3) | 8.3–24.9 | ||
| Total | 320 | 11.0(3.9) | 3.3–25.5 | 1.7(0.6) | 0.7–5.0 | 14.7(3.4) | 4.5–24.9 | ||
| KO | EN | 126 | 18.6(3.6) | 15.0–30.0 | 1.5(0.3) | 0.7–3.0 | 18.5(3.3) | 12.0–27.0 | |
| SP | 28 | 10.9(1.8) | 6.0–13.5 | 2.2(1.0) | 0.7–4.5 | 14.1(4.3) | 4.5–22.5 | ||
| MP | 4 | 9.7(1.5) | 7.5–10.5 | 2.2(0.9) | 1.5–3.0 | 19.9(3.3) | 15.0–22.5 | ||
| RF | 2 | 4.5(0.0) | 4.5–4.5 | 4.5(0.0) | 4.5–4.5 | 12.7(7.4) | 7.5–18.0 | ||
| Total | 160 | 16.9(4.8) | 4.5–30.0 | 1.7(0.7) | 0.7–4.5 | 17.7(3.9) | 4.5–27.0 | ||
| Kin | EN | 60 | 17.1(2.7) | 15.0–24.0 | 1.6(0.5) | 0.7–3.0 | 15.8(2.4) | 10.5–22.5 | |
| SP | 92 | 10.8(2.0) | 4.5–13.5 | 1.6(0.7) | 0.7–4.5 | 12.6(2.1) | 7.5–19.5 | ||
| MP | 8 | 7.9(1.6) | 6.0–10.5 | 1.5(0.0) | 1.5–1.5 | 17.2(3.0) | 12.0–22.5 | ||
| Total | 160 | 13.0(3.9) | 4.5–24.0 | 1.6(0.6) | 0.7–4.5 | 14.0(2.8) | 7.5–22.5 | ||
| +HASPB | EN | 32 | 16.0(0.8) | 14.9–16.6 | 1.6(0.4) | 0.8–2.5 | 16.8(2.0) | 11.6–19.9 | |
| SP | 102 | 11.0(1.8) | 6.6–13.3 | 1.6(0.3) | 0.8–3.3 | 13.4(2.2) | 6.6–19.9 | ||
| MP | 23 | 7.4(1.4) | 5.0–10.0 | 1.7(0.0) | 1.7–1.7 | 15.8(2.2) | 10.0–19.9 | ||
| RF | 3 | 5.0(2.9) | 3.3–8.3 | 3.9(0.9) | 3.3–5.0 | 7.2(2.5) | 5.0–10.0 | ||
| Total | 160 | 11.4(3.1) | 3.3–16.6 | 1.6(0.5) | 0.8–5.0 | 14.3(2.8) | 5.0–19.9 | ||
| +SHERP | EN | 98 | 19.4(2.9) | 14.9–29.9 | 1.6(0.4) | 0.8–3.3 | 19.1(2.8) | 13.3–30.0 | |
| SP | 61 | 11.1(1.8) | 8.3–13.3 | 1.5(0.4) | 0.8–2.5 | 13.9(2.5) | 6.6–19.9 | ||
| MP | 1 | 8.3 | 0.8 | 16.6 | |||||
| Total | 160 | 16.2(4.8) | 8.3–29.9 | 1.6(0.4) | 0.8–3.3 | 17.1(3.7) | 6.6–30.0 | ||
| 12 | FVI | EN | 31 | 15.6(1.0) | 15.0–18.3 | 1.9(0.7) | 0.7–4.5 | 14.9(2.2) | 10.5–19.5 |
| SP | 244 | 9.9(2.2) | 4.5–13.5 | 1.8(0.8) | 0.7–6.0 | 11.3(2.9) | 0.0–18.3 | ||
| MP | 40 | 6.6(1.4) | 4.5–9.0 | 1.6(0.5) | 0.8–3.3 | 15.1(3.3) | 9.0–22.5 | ||
| RF | 5 | 6.5(1.4) | 5.0–7.5 | 5.0(0.6) | 4.5–6.0 | 5.8(5.5) | 0.0–11.6 | ||
| Total | 320 | 10.0(2.9) | 4.5–18.3 | 1.8(0.9) | 0.7–6.0 | 12.0(3.4) | 0.0–22.5 | ||
| KO | EN | 105 | 17.2(2.3) | 15.0–22.5 | 1.6(0.6) | 0.7–3.0 | 15.1(2.3) | 9.0–22.5 | |
| SP | 54 | 11.7(1.7) | 7.5–13.5 | 1.9(0.8) | 0.7–3.0 | 11.9(3.1) | 6.0–21.0 | ||
| RF | 1 | 7.5 | 4.5 | 9.0 | |||||
| Total | 160 | 15.3(3.4) | 7.5–22.5 | 1.7(0.7) | 0.7–4.5 | 14.0(3.0) | 6.0–22.5 | ||
| Kin | EN | 82 | 19.2(4.1) | 14.9–28.2 | 1.7(0.4) | 0.8–3.3 | 18.7(4.5) | 11.6–28.2 | |
| SP | 68 | 11.9(1.7) | 6.6–13.3 | 1.9(0.6) | 0.8–3.3 | 13.4(2.4) | 8.3–19.9 | ||
| MP | 8 | 8.3(1.5) | 5.0–10.0 | 2.2(0.8) | 1.7–3.3 | 17.6(4.2) | 10.0–24.9 | ||
| RF | 2 | 8.3(4.7) | 5.0–11.6 | 5.0(0.0) | 5.0–5.0 | 16.6(2.3) | 14.9–18.3 | ||
| Total | 160 | 15.4(5.0) | 5.0–28.3 | 1.8(0.6) | 0.8–5.0 | 16.4(4.5) | 8.3–28.2 | ||
| +HASPB | EN | 14 | 15.9(1.1) | 15.0–18.3 | 1.9(0.6) | 0.8–3.3 | 15.8(1.1) | 14.9–18.3 | |
| SP | 124 | 10.6(2.0) | 6.6–13.3 | 1.7(0.4) | 0.8–3.3 | 13.0(2.4) | 6.6–19.9 | ||
| MP | 21 | 7.1(1.5) | 5.0–10.0 | 1.8(0.5) | 1.7–3.3 | 14.9(2.5) | 10.0–19.9 | ||
| RF | 1 | 5.0 | 3.3 | 8.3 | |||||
| Total | 160 | 10.6(2.8) | 5.0–18.3 | 1.7(0.5) | 0.8–3.3 | 13.4(2.6) | 6.6–19.9 | ||
| +SHERP | EN | 97 | 18.2(2.1) | 14.9–23.2 | 1.8(0.4) | 0.8–3.3 | 17.8(2.1) | 11.6–23.2 | |
| SP | 60 | 11.3(1.9) | 8.3–13.3 | 1.7(0.6) | 0.8–3.3 | 13.0(2.4) | 8.3–19.9 | ||
| MP | 2 | 5.0(0.0) | 5.0–5.0 | 2.5(1.2) | 1.7–3.3 | 11.6(2.3) | 10.0–13.3 | ||
| RF | 1 | 6.6 | 6.6 | 16.6 | |||||
| Total | 160 | 15.4(4.2) | 5.0–23.2 | 1.8(0.6) | 0.8–6.6 | 15.9(3.2) | 8.3–23.2 | ||
Promastigotes from gut smears were measured by light microscopy with an oil-immersion objective.
EN, elongated nectomonads; SP, short promastigotes; MP, metacyclic promastigotes; RF, round forms.