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. 2010 Nov 10;70(2):366–372. doi: 10.1136/ard.2010.133587

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Neutralising interleukin 6 (IL-6) antibody decreases the effects of systemic sclerosis (SSc) serum on endothelial cell activation. Human dermal microvascular endothelial cells were cultured with healthy control neutrophils for 24 h in the presence of 25% healthy control or SSc serum. Neutralising IL-6 rabbit polyclonal antibody was added at a dilution of 1:400. Apoptosis was measured using annexin V-FITC and E-selectin expression was measured using an allophycocyanin-conjugated monoclonal antibody. (A) Mean fluorescence intensity (MFI) measured by confocal microscopy and expressed as a fold change in mean fluorescence corrected to media alone (Rel MFI) (±SD, n=11). (B) Changes in E-selectin expression in individual control and SSc pairs (n=11). The addition of neutralising IL-6 antibodies to the co-cultures resulted in a decrease in the effect of SSc serum towards control levels.