Figure 3.

Regulation of expression of the histamine receptor H₄R in disease and by inflammatory cytokines. (a) Basal H₄R expression does not vary in different disease groups [psoriasis, atopic dermatitis (AD) and healthy controls, eight individual measurements and their mean are depicted]. (b–d) PBMC were stimulated for 48 hr with 20 ng/ml interferon-γ (IFN-γ) and H₄R expression on slanDC was determined flow cytometrically. The relative mean fluorescence intensity of non-stimulated cells versus IFN-γ-stimulated cells is shown for 6-sulpho LacNAc dendritic cells (slanDC) isolated from AD patients (b), healthy controls (c) and psoriasis patients (d). (The relative expression levels of H₄R were calculated as follows: specific staining times hundred divided by isotype staining.) **P < 0·005.