Figure 1.

Kinetics of p38 activation in cytokine-activated T (Tck) and anti-CD3 activated T (Ttcr) cells. CD4⁺ CD45RO⁺ T cells were stimulated with interleukin-2 (IL-2)/IL-6/tumour necrosis factor-α (TNF-α) or plate-bound anti-CD3. Cell lysates were collected at the indicated time. Activity of p38 was determined by phosphorylatation of its substrate mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2) in the kinase assay described (upper panel) and was measured by densitometer analysis and plotted as percentage of maximum response (lower panel). One representative experiment of three independent experiments is shown.