Figure 3.

Native Expression and Localization of Pta LBD1.

(A) Relative expression of LBD1 in different plant organs and tissues, as determined by RT-PCR: apex (A), leaf (L), whole internodal sections of stems undergoing predominantly primary growth (PSt), whole internodal sections of stems undergoing secondary growth (SSt), phloem (Ph), xylem (Xy), and root (R). See Methods for more sampling details. Data represent relative expression (mean ± se) from three independent biological replicates and are normalized to the expression of a ubiquitin loading control gene.

(B) and (C) In situ RT-PCR localization of LBD1 in stem sections from the 10th internode. The inset in (C) represents a higher magnification of the sector positioned to the right of the double-arrowhead line.

(D) and (E) No signal was detected in the negative control (-RT). Red arrowheads and double-arrowhead line demark the cambial zone on (B) and (C), respectively.

CZ, cambium zone; PhF, phloem fiber; Ph, phloem; Xy, xylem. Bars = 100 μm in (B) and (D), 10 μm in (C) and (E), and 2 μm in the inset in (C).