The prr7 prr9 Double Mutant Overcompensates.
Seedlings carrying a CCA1pro:LUC or TOC1pro:LUC circadian reporter (Salomé and McClung, 2005) were entrained to light-dark cycles for 7 to 8 d at 23°C. Luciferase activity was then recorded from day 10 at 12, 16, 22, or 30°C as described in Methods. All data (luciferase activity and circadian periods) are shown as mean ± se (s.e.m.; n = 12 to 24).
(A) Mean circadian traces for CCA1pro:LUC activity in Col-2 and prr7 prr9 at 12, 22, and 30°C.
(B) Mean circadian period of the CCA1pro:LUC reporter in Col-2 and prr7 prr9 as a function of temperature.
(C) Temperature compensation behavior of prr5 prr7 and prr7 prr9 double mutants.
(D) Temperature compensation behavior of the gi-1 (weak) and gi-201 (strong) mutant alleles.
(E) Temperature compensation behavior of the long period mutants arr3 arr4 and ztl-4.
(F) Temperature compensation behavior of the core clock component mutants lhy-20 and toc1-101.
(G) Temperature compensation behavior of the flc-3 mutant in the Col-0 background.