Figure 6.

Loss of PRR7 and PRR9 Results in the Accumulation of Morning Loop Genes but Not Evening Loop Components.

(A) Normal expression of the clock genes CCA1, LHY, and TOC1 is restored in the prr7 prr9 mutant at 12°C.

(B) prr7 prr9 accumulates more CCA1 and LHY mRNA at higher temperatures than Col-0. Expression values were integrated over the whole time course.

(C) HSP70 expression is under the control of the clock. LHY expression is shown as control for a strongly rhythmic gene. Data extracted from Gould et al. (2006) and shown as mean ± sd (n = 3).

(D) Induction of HSP70 expression by ambient temperature is maintained in prr7 prr9. Left panel: expression values from publicly available microarray data (Gould et al., 2006). Middle and right panels: HSP70 expression determined by quantitative PCR in Col-0 and prr7 prr9 at Zeitgeber Time 72 (ZT72).

Expression values are shown for one biological replicate as mean ± sd (n = 2).