Figure 9.

AMT1;1 and AMT1;3 Are Differently Regulated under Localized Ammonium Supply.

(A) Protein accumulation of AMT1;1 and AMT1;3 in roots from the LN compartment or the HN compartment supplemented with 0.8 mM ammonium. Wild-type plants were grown on vertically split agar plates for 15 d. Gel blots from microsomal membrane fractions were probed with anti-AMT1;1, anti-AMT1;3, and anti-DET3.

(B) Localization of AMT1;1 and AMT1;3. AMT1;1 promoter:AMT1;1 gene:GFP and AMT1;3 promoter:AMT1;3 gene:GFP plants were grown on vertically split agar plates, and GFP-derived fluorescence on the HN compartment was observed under a confocal microscope. The top and bottom panels show first-order lateral root tips and the differentiation zone of second-order lateral roots with third-order lateral root primordia, respectively. Bars = 50 μm.