Figure 3.

AQC1 Encodes TPST.

(A) Cloning of the AQC1/TPST gene. Black boxes represent exons, lines between black boxes represent introns, and white boxes represent the 5′ and 3′ noncoding regions. Arrowheads mark the T-DNA insertion sites or the mutation site of different alleles. Chr., chromosome.

(B) Schematic representation of the AQC1/TPST protein, modified from Moore (2009). SP, signal peptide; TM, transmembrane helix.

(C) Phylogenetic analysis of AQC1/TPST and its orthologs from other plant species. The node labels indicate the proportion of trees in the posterior distribution containing the node. Sequences were from Ostreococcus lucimarinus (Ol TPST), Selaginella moellendorffii (Sm TPST), Physcomitrella patens (Pp TPST), Arabidopsis thaliana (At TPST), Arabidopsis lyrata (Al TPST), Vitis vinifera (Vv TPST), Glycine max (Gm TPST), Carica papaya (Cp TPST), Populus trichocarpa (Pt TPST), Sorghum bicolor (Sb TPST), Brachypodium distachyon (Bd TPST), and Oryza sativa (Os TPST). The sequences and alignment used are presented in Supplemental Data Set 1 online.